cd8a mouse 168er Search Results


rat monoclonal anti mouse cd8a 168er  (fluidigm)
95
fluidigm rat monoclonal anti mouse cd8a 168er
(A) Representative hematoxylin and eosin staining of skin tissues from mice fed the LFD, fish oil HFD, or cocoa butter diet at different magnifications (red arrows: infiltrating inflammatory cells). Scale bars, 100 μm. (B and C) Flow cytometric analysis of immune cell phenotype in the dermis of mice on different diets for 3 months. A cell population with strong autofluorescence (red trapezoid gate) was specifically accumulated in the dermis of mice fed the fish oil HFD (B). Multichannel signals of the autofluorescent cells were analyzed using a BD Fortessa flow cytometer (C). (D–F) Autofluorescent cells in the dermis of fish oil HFD-fed mice were purified using a BD FACSAria II flow sorter and stained with a panel of metal-tagged CyTOF antibodies. Uniform manifold approximation and projection (UMAP) was used to visualize and identify immune cell populations in unsorted dermal cells (D) and sorted autofluorescent dermal cells (E). Individual surface marker signatures in the CyTOF panel are shown in (F). (G and H) Representative IHC images of F4/80 + macrophages (brown staining, G) and <t>CD8</t> + T cells (brown staining, H) in the skin of mice fed the LFD, fish oil HFD, or cocoa butter HFD. Scale bars, 100 μm. See also . These in vitro experiments were repeated with at least three biological replicates.
Rat Monoclonal Anti Mouse Cd8a 168er, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat monoclonal anti mouse cd8a 168er/product/fluidigm
Average 95 stars, based on 1 article reviews
rat monoclonal anti mouse cd8a 168er - by Bioz Stars, 2026-03
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(A) Representative hematoxylin and eosin staining of skin tissues from mice fed the LFD, fish oil HFD, or cocoa butter diet at different magnifications (red arrows: infiltrating inflammatory cells). Scale bars, 100 μm. (B and C) Flow cytometric analysis of immune cell phenotype in the dermis of mice on different diets for 3 months. A cell population with strong autofluorescence (red trapezoid gate) was specifically accumulated in the dermis of mice fed the fish oil HFD (B). Multichannel signals of the autofluorescent cells were analyzed using a BD Fortessa flow cytometer (C). (D–F) Autofluorescent cells in the dermis of fish oil HFD-fed mice were purified using a BD FACSAria II flow sorter and stained with a panel of metal-tagged CyTOF antibodies. Uniform manifold approximation and projection (UMAP) was used to visualize and identify immune cell populations in unsorted dermal cells (D) and sorted autofluorescent dermal cells (E). Individual surface marker signatures in the CyTOF panel are shown in (F). (G and H) Representative IHC images of F4/80 + macrophages (brown staining, G) and CD8 + T cells (brown staining, H) in the skin of mice fed the LFD, fish oil HFD, or cocoa butter HFD. Scale bars, 100 μm. See also . These in vitro experiments were repeated with at least three biological replicates.

Journal: Cell reports

Article Title: Consumption of fish oil high-fat diet induces murine hair loss via epidermal fatty acid binding protein in skin macrophages

doi: 10.1016/j.celrep.2022.111804

Figure Lengend Snippet: (A) Representative hematoxylin and eosin staining of skin tissues from mice fed the LFD, fish oil HFD, or cocoa butter diet at different magnifications (red arrows: infiltrating inflammatory cells). Scale bars, 100 μm. (B and C) Flow cytometric analysis of immune cell phenotype in the dermis of mice on different diets for 3 months. A cell population with strong autofluorescence (red trapezoid gate) was specifically accumulated in the dermis of mice fed the fish oil HFD (B). Multichannel signals of the autofluorescent cells were analyzed using a BD Fortessa flow cytometer (C). (D–F) Autofluorescent cells in the dermis of fish oil HFD-fed mice were purified using a BD FACSAria II flow sorter and stained with a panel of metal-tagged CyTOF antibodies. Uniform manifold approximation and projection (UMAP) was used to visualize and identify immune cell populations in unsorted dermal cells (D) and sorted autofluorescent dermal cells (E). Individual surface marker signatures in the CyTOF panel are shown in (F). (G and H) Representative IHC images of F4/80 + macrophages (brown staining, G) and CD8 + T cells (brown staining, H) in the skin of mice fed the LFD, fish oil HFD, or cocoa butter HFD. Scale bars, 100 μm. See also . These in vitro experiments were repeated with at least three biological replicates.

Article Snippet: Rat monoclonal anti-mouse CD8a - 168Er , Fluidigm , Cat#3168003B; RRID:AB_2811241.

Techniques: Staining, Flow Cytometry, Purification, Marker, In Vitro

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Consumption of fish oil high-fat diet induces murine hair loss via epidermal fatty acid binding protein in skin macrophages

doi: 10.1016/j.celrep.2022.111804

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rat monoclonal anti-mouse CD8a - 168Er , Fluidigm , Cat#3168003B; RRID:AB_2811241.

Techniques: Purification, Recombinant, Activation Assay, SYBR Green Assay, Reverse Transcription, Detection Assay, Enzyme-linked Immunosorbent Assay, Selection, Software